Molecular biology
a branch of science thatAllows to modify
Allows to modify
a microorganism
Thanks to this technology we are able to improve the productivity of a secondary metabolite, rebuild a metabolic pathway switching on or off particular genes (metabolic engineering), improve the characteristic of an enzyme, or insert in a host a complete cluster of your interest.
Polymerase chain reaction (PCR) is a key tool, that can be considered as the most powerful tool currently available in enzyme modification
Indeed, it is possible to generate a library of mutants that could be tested for the desired characteristics:
maximum yields, higher stability, low inhibition, high efficiency and low side activity.
Identification and optimization of gene sequences
Homology modeling, docking and molecular dynamic
Heterologous expression of proteins and peptides in bacteria and yeast
Microbial genotyping
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